It contains 0.31 ml of 32 mg/ml liposome composed of DOPC: cholesterol : DSPE-PEG2000 (mass ratio: 7.5 : 1 : 2.5) and 1.69 ml of 5 % maleic acid (SMA) 2:1 copolymer in 10 mM Tris-HCl, pH 7.6. To make the 30 nm nanodisc, mix the two reagents and incubate the mixture at room temperature overnight.