Type II DNA topoisomerases including topoisomerase II and topoisomerase IV hydrolyze ATP as the source of molecular energy to carry out DNA supercoiling and DNA decatenation reactions. Inhibition of the ATPase activity of topoisomerases by compounds such as novobiocin blocks their biological functions.   Therefore, the topoisomerase ATPase assay can be used for high-throughput screen of topoisomerase inhibitors.  The Topoisomerase ATPase Assay Kit is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm.

E. coli gyrase ATPase assay Kit Plus (Catalog No.  T2A-100KE)

S. aureus gyrase ATPase assay Kit Plus (Catalog No.  T2A-100KS)

P. aeruginosa Gyrase ATPase assay Kit Plus (Catalog No. T2A-100KP)

E. coli Topo IV ATPase Assay Kit Plus (Catalog No.  T4A-100KE)

S. aureus Topoisomerase IV ATPase Assay Kit Plus (Catalog No.  T4A-100KS)

P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (Catalog No. T4A-100KP)

S. pneumoniae Topoisomerase IV ATPase Assay Kit Plus (Catalog No. T4A-100KN)

References:

Kalsy M. et al, The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilms.  Biofilms and Microbiomes volume 6, Article number: 6 (2020).