DNA decatenation is an essential process during DNA replication in the cells. The DNA decatenation reaction was carried out by topoisomerase IV (the parC-parE complex) in bacteria.  The DNA decatenation reaction converts the concatenated DNA into decatenated DNA.   TheGel-Based E. coli Topoisomerase IV DNA Decatenation Assay is based on the principle that the decatenated DNA and concatenated DNA are separated by agarose gel electrophoresis.  Fluorescence-based DNA decatenation assays in a 96-well pate format are also available for high throughput screening of gyrase inhibitors.  For more information of the high throughput DNA decatenation assays, please visit the website at http://www.profoldin.com/topo_iv_assays.html.

The Gel-Based E. coli Topoisomerase IV DNA Decatenation Assay Kit Plus (Catalog No. GDDA100KE) includes all the reagents for 100 samples in gel-based assays of E. coli topoisomerase IV DNA decatenation activity.  It includes 300 µl of 10 x Buffer, 205 µl of 60 mg/ml concatenated DNA (kDNA), 205 µl of 10 mM ATP,  550 µl of  5 x Gel loading buffer and 22 µl of 10 µM E.coli topoisomerase IV (the parC-parE complex).