This product includes 480 ul of 10 x Buffer T1, 405 ul of 10 x supercoiled plasmid DNA, 8 ul of 4500 x Dye H19, 3000 ul of 10 x H19 dilution buffer and 42 ul of 100 x E. coli topoisomerase I. It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format