This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x Enzyme Substrate, 0.035 ml of 100 x E. coli D-Ala:D-Ala ligase (200 nM) and 5 ml of Dye for phosphate detection.