This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Biotin, 0.170 ml of 100 x hydroxylamine, 0.170 ml of 100 x ATP, 0.170 ml of 100 x E. coli BirA (5000 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml) and 25 ml of Dye for phosphate detection.