This product includes 600 ul of 10 x assay buffer, 500 ul of 10 x concatenated DNA, 110 ul of 10 mM ATP, 8 ul of 1000 x S. aureus topo IV, 600 ul of 0.4 M EDTA, 260 ul of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.