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Human DNA polymerase beta assay
DNA polymerase beta play key roles in the base excision repair (BER) process. It fills the small gaps (1 – 6 bases) of one DNA stand using the complementary strand as a template. Human DNA polymerase beta is an anti-cancer target. The human DNA polymerase beta assay is based on detection of the DNA polymerase activity in the presence of a DNA template and dNTPs. The Assay Kit (catalog number DPB100K) is to measure the activity of DNA polymerase beta and test DNA polymerase beta inhibitors.
Each Human DNA Polymerase Beta Assay Kit (Catalog No: DPB100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template (3 µM), 33 µl of 100 x dNTP mix (10 mM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.
Each Human DNA Polymerase Beta assay Kit Plus (Catalog No: DPB100KE) includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template (3 µM), 33 µl of 100 x dNTP mix (10 mM), 33 µl of 100 x human DNA polymerase beta, 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.
References:
- Merlo S et al, Identification of 5‑Methoxyflavone as a Novel DNA Polymerase-Beta Inhibitor and Neuroprotective Agent against Beta-Amyloid Toxicity. J. Nat. Prod. 78, 2704−2711 (2015).
- Maldonado E et al, Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form, Parasitol Res (2015) 114:1313–1326.
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