Many peptide derivatives or modified peptides have become peptide-based drugs for anti-cancer, anti-infection and other therapeutic applications. Peptide-based drugs often show higher specificity and lower side effects than regular small molecule-based drugs.  The Peptide Derivative Assay Kit (Catalog number PEPD200) is designed for concentration measurement of various modified peptides in a DMSO solution.  The assay is based on increase of fluorescence at 535 nm generated by binding of Dye C33 with peptides. The assay sensitivity is typically between 0.01 mg/ml and 0.1 mg/ml depending on the structure of the peptide derivatives.  The assay is not compatible with amino acids, native peptides, thiol compounds such as DTT or metal chelators such as EDTA.

The Peptide Derivative Assay Kit (Catalog number PEPD200) includes 1 ml of 10 x C33 dye for 200 assays using 96-well plates.  Cuvettes may also be used for the assay.