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Bacterial DNA Primases – RNA primer synthesis assay
The bacterial DNA primase synthesizes RNA primers at the DNA replication fork where the DNA helicase (DnaB) unwinds the double strand DNA. The Bacterial DNA Primase Assay Kits are based on measurement of the RNA primers synthesized by the DNA primase in the presence of the DNA temperate and DNA helicase. The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA primase inhibitors.
Each kit includes the assay buffer and DNA template for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format. Enzyme is not included.
The E. coli DNA primase assay kit including the E. coli primase-helicase complex.
Each kit includes the assay buffer and DNA template for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format. Enzyme is not included.
The H. influenzae DNA primase assay kit including the H. influenzae primase-helicase complex.
Each kit includes the assay buffer and DNA template for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format. Enzyme is not included.
The S. aureus DNA primase assay kit including the S. aureus primase.
Each kit includes the assay buffer and DNA template for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format. Enzyme is not included.
The S. pneumoniae DNA primase assay kit including the S. pneumoniae primase.
References:
1. Chiriac AI et al, Mode of action of closthioamide: the first member of the polythioamide class of bacterial DNA gyrase inhibitors. J. Antimicrob. Chemother. 70 (9): 2576-2588 (2015).
2. Lacriola CJ et al, Inhibition of DNA replication in Staphylococcus aureus by tegaserod. The Journal of Antibiotics. 70, 918–920 (2017).
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