|
Human topoisomerase I – DNA relaxation assay
Human topoisomerase I relaxes supercoiled plasmid DNA to form relaxed DNA. The Human DNA topoisomerase I assay is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases. The change of fluorescence intensity is used to measure the relaxation reaction.
References:
1. Carlos Urda et al, Bistratamides M and N, Oxazole-Thiazole Containing Cyclic Hexapeptides Isolated from Lissoclinum bistratum Interaction of Zinc (II) with Bistratamide K. Marine Drugs, 15(7), 209 (2017).
2. Abdellatif, K. R.A. et al, Design, Synthesis and Cytotoxicity Evaluation of New 3, 5-Disubstituted-2-Thioxoimidazolidinones. Anti-Cancer Agents in Medicinal Chemistry, Volume 18, 573 (2018).
3. Abdellatif K.R.A. Thiohydantoin derivatives incorporating a pyrazole core: Design, synthesis and biological evaluation as dual inhibitors of topoisomerase-I and cycloxygenase-2 with anti-cancer and anti-inflammatory activities. Bioorganic Chemistry, Volume 91, 103132 (2019).
4. Costa M. el al, Two new spongian diterpene analogues isolated from the marine sponge Acanthodendrilla sp. Natural Product Research, Volume 34 - Issue 8, Pages 1053-1060 (2020).
|
