Thymidylate kinase is an important enzyme in the dTTP synthesis pathway for DNA synthesis. The thymidylate kinase activity is higher in the tumor than in the corresponding normal human tissue.  The function of this enzyme is to catalyze the phosphorylation of dTMP using ATP to form dTDP and ADP.  The thymidylate kinase assay is based on measurement of ADP generated from the kinase reaction.  The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes.  

Human Thymidylate Kinase Assay Kit Plus-500 (Catalog Number:  HTMK500KE)

References:

Choi JY et al, Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation, J Med Chem.  January 26; 55(2): 852–870 (2012).