Human DNA Polymerase Alpha (Pol α) is responsible for the initiation of DNA replication at origins of replication The Pol α enzyme consists of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2.  The human DNA polymerase alpha assay is based on measurement of the DNA molecules synthesized by the DNA polymerase.  The assay is performed in a 384-well or 96-well plate format. The assay can be used for detection of DNA polymerase alpha activity and high throughput screen of human DNA polymerase alpha inhibitors.  

For colorimetric assay detecting phosphate, please use Human DNA Polymerase Alpha Pyrophosphate Assay Kit Plus (Catalog No.  HDPA100KEP).

The Human DNA Polymerase Alpha Assay Kit (Catalog No.  HDPA100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP  mix, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. The kit does not include the enzyme.

The Human DNA Polymerase Alpha Assay Kit Plus (Catalog No.  HDPA100KE1) includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP  mix, 30 µl of 100 x human DNA polymerase alpha,  1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.