The Bacteriophage T4 DNA Ligase Assay Kit (Catalog number T4L100K) includes 500 ul of 10 x buffer, 44 ul of 100 x DNA substrate, 44 ul of 100 x ATP (2 mM), 21 ml of Reagent T and 220 ul of 10 x dye for 100 assays. The kit does not include ligase.