It includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 30 ul of 100 x DNA, 30 ul of 100 x dNTP,  15 ul of 200 x human DNA polymerase alpha catalytic domain, 30 ul of 100 x pyrophosphatase (PPase) and 6 ml of Dye.