The kit includes all the assay kit components for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA (20 µM), 33 µl of 100 x ATP (25 mM), 33 µl of 100 x human DNA polymerase theta N-terminal domain and 5 ml of MPA3000 dye.