This product includes all the reagents for 100 samples in gel-based assays of E. coli topoisomerase IV DNA decatenation activity.  It includes 300 µl of 10 x Buffer, 205 µl of 60 mg/ml concatenated DNA (kDNA), 205 µl of 10 mM ATP,  550 µl of  5 x Gel loading buffer and 22 µl of 10 µM E.coli topoisomerase IV (the parC-parE complex).