This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer,0.170 ml of 100 x NA, 0.170 ml of 100 x PRPP, 0.170 ml of 100 x ATP, 0.170 ml of 100 x S. aureus PncB (5000 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection