This product includes 500 ul of 10 x Buffer T2, 410 ul of 10 x relaxed DNA, 8 ul of 4500 x H19 dye, 450 ul of 10 x ATP, 43 ul 7.5 uM S. aureus gyrase, 820 ul of 2 M potassium glutamate and 3000 ul of 10 x H19 dilution buffer. It is for 100 assays in a 96-well plate format.