This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer,0.170 ml of 100 x MEP, 0.170 ml of 100 x CTP, 0.170 ml of 100 x P. aeruginosa IspD (200 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection.